How long does te buffer last

Although oligos remain stable through repeated freeze-thaw cycles, repeated exposure to the environment could inadvertently introduce nucleases.

To prevent contamination of stock solutions, we recommend aliquoting oligos before storage. While we have not extensively tested long term stability of our full catalog of modifications using different storage conditions, we do expect most modified oligos to possess similar storage characteristics as unmodified oligos.

Freezing is optimal when storing modified oligos for long durations, and IDTE buffer will help preserve stability at temperatures above freezing. Short term exposure to UV light, ambient lab light, or ozone should not affect the function of fluorophore-modified oligos. Avoiding RNase contamination is crucial to maintaining RNA oligo stability, as even the slightest exposure to RNase can cause substantial degradation.

The preceding facts and data were generated during a multi-year longitudinal study of oligo stability. Our scientific application specialists are readily available to answer any further questions or to provide guidance on oligo handling and experimental setup.

Contact them at applicationsupport idtdna. Innate Immun. Farrell RE Chapter 2: Creating a ribonuclease-free environment. Boston: Academic Press. Verified by mass spectrometry. Include mixed bases, modifications. Ready for gene construction, genome editing, PCR, or sequencing.

Delivered purified and normalized. Indispensable for linking an oligo to another molecule, providing resistance to nuclease degradation, and more. Use these programs to calculate T m , identify secondary structure, optimize codon use, select siRNA, and more. All rights reserved.

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Microbiology Reading, England 1 , — Wen, C. Guillo, J. Ferrance, J. Anal Chem 78 5 , — Pure water lacks buffering capacity and an acidic pH may lead to DNA hydrolysis. An EU workshop on Biobanks ref. Similarly, the UK Biobank ref. We hope these best practices will help you optimize the long term storage of all your purified Oragene samples. High-throughput DNA purification. Modern Drug Discovery. January , Version 1. UK Biobank.

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